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Risk Assessment -Activities

Sea Water Quality Criteria

  • Collection, transportation, acclimation and maintenance of marine organisms for toxicity bioassay experiments.
  • Toxicity bioassay experiments on marine organisms for metals(Cd, Cu, Pb, Zn, Hg, As, Cr, Ni, Se), organics (monocrotophos, chlorpyrifos) and hydrocarbons (Anthracene and Benzo-a-pyrene)
  • Derivation of toxicity values: Median lethal Concentration (LC50), Lowest Observed Effect Concentration (LOEC), No Observed Effect Concentration (NOEC) and Chronic values (CV).
  • Measurements of the metals and pesticide of the experimental samples using the analytical instruments like Gas Chromatography (GC) fitted with FPD and FID Detectors, Atomic Absorption spectrophotometer.
  • Histology: Histological observations in the tissue sections of the matine organisms after exposure to the sublethal concentrations of the selected metal/pesticide/hydrocarbons
  • Biochemistry: Biochemical enzyme activities by using Multimode Reader and Native PAGE (Polyacrylamide Gel Electrophoresis).
  • Derivation of Sea Water Quality Criteria based on the Acute and Chronic toxicity values of marine organisms.

 

Ecological Risk Assessment

  • Monitoring of metals, PAHs, TPH in water and sediment samples of Ennore, Uppanar, and Vellar Estuaries
  • Monitoring of water quality parameters and nutrients from the samples of Ennore, Uppanar, and Vellar Estuaries
  • Qualitative and quantitative analysis of the benthos from the sediment samples of selected estuarine transects.
  • Comprehensive sediment quality assessment through integrated approach by using sediment toxicity bioassay, measurement of contaminants and benthic biodiversity assessment.
  • Collection and optimization of monoculture of diatoms, copepods, Polychaete larvae and barnacles from mixed culture for toxicity bioassays.
  • Microcosm experiments on marine diatoms, copepods, and larvae of polychaete and barnacles for toxicity sediment extracts collected from Ennore, Uppanar, and Vellar Estuaries.
  • Derivation of environmental quality indices for the assessment of extent of damage due to metal/TPH/PAH pollutants.
  • Field based in situ mesocosm experiments on selected marine organisms at Ennore estuary for the risk assessment of metals of concern.